Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Andersen LE[original query] |
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Clinical surveillance and evaluation of suspected Ebola cases in a vaccine trial during an Ebola epidemic: The Sierra Leone Trial to Introduce a Vaccine Against Ebola
Conteh MA , Goldstein ST , Wurie HR , Gidudu J , Lisk DR , Carter RJ , Seward JF , Hampton LM , Wang D , Andersen LE , Arvay M , Schrag SJ , Dawson P , Fombah AE , Petrie CR , Feikin DR , Russell JBW , Lindblad R , Kargbo SAS , Samai M , Mahon BE . J Infect Dis 2018 217 S33-s39 Clinical Trials Registration: ClinicalTrials.gov [NCT02378753] and Pan African Clinical Trials Registry [PACTR201502001037220]. |
Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses.
Santiago GA , Vazquez J , Courtney S , Matias KY , Andersen LE , Colon C , Butler AE , Roulo R , Bowzard J , Villanueva JM , Munoz-Jordan JL . Nat Commun 2018 9 (1) 1391 The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses. To facilitate detection of ZIKV infections, and differentiate these infections from DENV and CHIKV, we developed the Trioplex real-time RT-PCR assay (Trioplex assay). Here, we describe the optimization of multiplex and singleplex formats of the assay for a variety of chemistries and instruments to facilitate global standardization and implementation. We evaluated the analytical performance of all Trioplex modalities for detection of these three pathogens in serum and whole blood, and for ZIKV in urine. The limit of detection for the three viruses and in different RNA-extraction modalities is near 10(3) genome copy equivalents per milliliter (GCE/mL). Simultaneous testing of more than one specimen type from each patient provides a 6.4% additional diagnostic sensitivity. Overall, the high sensitivity of the Trioplex assay demonstrates the utility of this assay ascertaining Zika cases. |
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